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Home  >  Journal list  >  Polymer Journal  >  Vol.49  No.11 (2017)  >  pp.789-798

Polymer Journal
<<Previous article Vol.49  No.11 (2017)   pp.789 - 798 Next article>>

Membranes based on carboxymethyl chitin as potential scaffolds for corneal endothelial transplantation

Wenhua Xu, Zheying Wang, Tong Li, Liping Wang, Wenhua Zhang, Ye Liang and Chengyu Liu
College of Medicine, Qingdao University, Qingdao, China
Wenhua Xu, Zheying Wang, Tong Li, Wenhua Zhang & Chengyu Liu
Key Laboratory, Department of Urology and Andrology, The Affiliated Hospital of Qingdao University, Qingdao, China
Liping Wang & Ye Liang


To overcome the worldwide shortage of donor corneas, transplantation of cultured corneal endothelial cells (CECs) as substitutes has been attempted in experimental studies to treat corneal endothelial dysfunction. In the present study, corneal endothelial scaffolds were prepared using carboxymethyl chitin (CMCT) or carboxymethyl chitosan (CMCTS) as the main ingredient and gelatin (gel) at a mass ratio of 20:1. The transmittances of the membranes were examined at different wavelengths (400, 500, 600, 700 and 800 nm). The cytotoxicity of the blend membranes was evaluated by MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide) assay. The properties of both membranes, including biodegradability, immunogenicity and toxicity, were determined by implantation experiments in Sprague-Dawley rats. Then the rabbit primary CECs were implanted onto the blended membranes to evaluate their cytocompatibility. Results indicated that both the CMCT/gel and CMCTS/gel blended membranes exhibited good transparency and cytocompatibility, with relatively low in vivo toxicity. However, the CMCT/gel membranes showed a higher degradation rate and milder immune response in vivo compared with the CMCTS/gel. The degradation time of the CMCT/gel membranes was <8 weeks in the subcutaneous tissues and 4 weeks in the skeletal muscles. Thus the CMCT/gel blended membrane could be a more promising candidate for constructing endothelial scaffold in corneal replacement.


Received: March 23, 2017 , Revised: June 29, 2017
Accepted: July 23, 2017 , Published online: September 13, 2017
© 2017 The Society of Polymer Science, Japan

NatureAbstract (Nature)


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