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Home  >  Journal list  >  Polymer Journal  >  Vol.41  No.6 (2009)  >  pp.486-491

Polymer Journal
<<Previous article Vol.41  No.6 (2009)   pp.486 - 491 Next article>>

Heterofunctional Interfaces Achieve Dual Protein Adsorption on Polyelectrolyte Multilayers

Heyun Shen1)2), Junji Watanabe1)2) and Mitsuru Akashi1)2)
1) Department of Applied Chemistry, Graduate School of Engineering, Osaka University
2) 21st Century COE for “Center for Integrated Cell and Tissue Regulation,” Osaka University

ABSTRACT:  Two types of polyelectrolyte multilayers were formed on both sides of a substrate by integrating an alternate adsorption process with an alternate drop-coating process. We used poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) as strong polyelectrolytes. Each side of the substrate was covered with each polyelectrolyte, PDDA and PSS, then heterofunctional interfaces were obtained. Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) and cytochrome c (cyt c) were used as model proteins for evaluating dual protein adsorption. The proteins FITC-BSA and cyt c exhibited anionic and cationic charges, respectively, in tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer solution (pH 7.4). From the results of protein adsorption from individual protein solutions, the FITC-BSA and cyt c proteins were adsorbed on the outermost surfaces of PDDA and PSS, respectively. Moreover, dual protein adsorption was achieved in the case of a protein mixture. This result indicated that polyelectrolyte multilayers could easily control the surface charge to regulate protein adsorption from the protein mixture. In summary, a heterofunctional interface prepared from polyelectrolyte multilayers achieved dual protein adsorption on each side of a substrate from a protein mixture.

Heterofunctional Interface/ Drop-Coating/ Polyelectrolyte Multilayer/ Dual Protein Adsorption

Published online: June 15, 2009
© Copyright, 2009 by The Society of Polymer Science, Japan

NatureAbstract (Nature)


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